研究業績

雑誌論文 図書 学会発表

　
◆ 雑 誌 論 文 ◆

I. 難治性慢性疼痛等のトランスレーショナル研究

１）慢性疼痛患者の身体、心理、認知的側面についての研究
  1. 水野泰行, 阿部哲也, 中井吉英.　慢性疼痛に対する集学的アプロ−チ−心療内科の立場から,　ペインクリニック, 28, 1589-1597, 2007.
  2. 中井吉英, 水野泰行, 阿部哲也.　システム論的な見方による難治性疼痛の予防と治療,　醫學のあゆみ,　223, 784-789, 2007.
  3. 阿部哲也, 中井吉英.　痛みの臨床心理学, 理学療法, 13, 23-27, 2006.
  4. 西山順滋, 中井吉英.　胸部の慢性疼痛,　痛みと臨床, 6, 264-271, 2006.
  5. 水野泰行, 中井吉英.　抑肝散が有効であった慢性疼痛の1例,　日本東洋心身医学研究, 21, 52-55, 2006.

２）報酬と嫌悪の神経メカニズム

  1. Yoshizawa, K., Nakao, K., Habiro, M., Hayashi, K., Kuwata, M., Uehara, N., Takashi, Y., Nakamura, K. and Tsubura, A.　Cerebromalacia with epilepsy and cortical blindness in a laboratory Japanese macaque (Macaca fuscata). Toxicol. Pathol., in press, 2010.
  2. Cools, R., Nakamura, K. and Daw, N.D. Serotonin and dopamine: Unifying affective, activational, and decision functions. Neuropsychopharmacol. Rev., in press, 2010.
  3. Bromberg-Martin, E.S., Hikosaka, O. and Nakamura, K. Coding of task reward value in the dorsal raphe nucleus. J. Neurosci., 30, 6262-6272, 2010.
  4. Nakamura, K., Matsumoto, M. and Hikosaka, O. Reward-dependent modulation of neuronal activity in the primate dorsal raphe nucleus. J. Neurosci., 28, 5331-5343, 2008.

３）癌性疼痛、帯状疱疹後神経痛患者に対する脊髄腔内ステロイド投与の臨床研究と治療評価のための標的タンパクの探索

  1. Taguchi, H., Oishi, K., Sakamoto, S. and Shingu, K. Intrathecal betamethasone for cancer pain in the lower half of the body: a study of its analgesic efficacy and safety. Br. J. Anaesth., 98, 385-389, 2007.
  2. Inada, T., Kushida, A., Sakamoto, S., Taguchi, H. and Shingu, K. Intrathecal betamethasone pain relief in cancer patients with vertebral metastasis: a pilot study. Acta Anaesthesiol. Scand., 51, 490-494, 2007.
  3. Sasaki, A., Mabuchi, T., Serizawa, K., Takasaki, I., Andoh, T., Shiraki, K., Ito, S. and Kuraishi, Y. Different roles of nitric oxide synthase-1 and -2 between herpetic and postherpetic allodynia in mice. Neuroscience, 150, 459-466, 2007.
  4. Soen, M., Minami, T., Tatsumi, S., Mabuchi, T., Fruta, K., Maeda, M., Suzuki, M. and Ito, S. A synthetic kainoid, (2S,3R,4R)-3-carboxymethyl-4-(phenylthio) pyrrolidine-2-carboxylic acid (PSPA-1) serves as a novel anti-allodynic agent for neuropathic pain. Eur. J. Pharmacol., 575, 75-81, 2007.

４）神経再生による難治性疼痛患者の診断・治療のための標的タンパクの探索

５）慢性疼痛の発症・維持機構の基礎研究

  1. Matsumura, S., Kunori, S., Mabuchi, T., Katano, T., Nakazawa, T., Abe, T., Watanabe, M., Yamamoto, T., Okuda-Ashitaka, E. and Ito, S. Impairment of CaMKII activation and attenuation of neuropathic pain in mice lacking NR2B phosphorylated at Tyr1472. Eur. J. Neurosci., 32, 798-810, 2010.
  2. Tada, H., Okano, H.J., Takagi, H., Shibata, S., Yao, I., Matsumoto, M., Saiga, T., Nakayama, K.I., Kashima, H., Takahashi, T., Setou, M. and Okano, H. Fbxo45, a novel ubiquitin ligase, regulates synaptic activity. J. Biol. Chem., 285, 3840-3849, 2010.
  3. Ohnishi, T., Matsumura, S. and Ito, S. Translocation of neuronal nitric oxide synthase to the plasma membrane by ATP is mediated by P2X and P2Y receptors. Mol. Pain, 5, 40-, 2009.
  4. Lu, J., Katano, T., Okuda-Ashitaka, E., Oishi, Y., Urade, S. and Ito, S. Involvement of S-nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide. Mol. Pain, 5, 58-, 2009.
  5. Katano, T., Furue, H., Okuda-Ashitaka, E., Tagaya, M., Watanabe, M., Yoshimura, M. and Ito, S. N-Ethylmaleimide-sensitive fusion protein(NSF) is involved in central sensitization in the spinal cord through GluR2 subunit composition switch after inflammation. Eur. J. Neurosci., 27, 3161-3170, 2008.
  6. Ohnishi, T., Okuda-Ashitaka, E., Matsumura, S., Katano, T., Nishizawa, M., and Ito, S. Characterization of signaling pathway for the translocation of neuronal nitric oxide synthase to the plasma membrane by PACAP. J. Neurochem., 105, 2271-2285, 2008.
  7. Moriuchi, H., Koda, N., Okuda-Ashitaka, E., Daiyasu, H., Ogasawara, K., Toh, H., Ito, S., Woodward D.F. and Watanabe, K. Molecular Characterization of a Novel Type of Prostamide/Prostaglandin F Synthase, Belonging to the Thioredoxin-like Superfamily. J. Biol. Chem., 283, 792-801, 2008.
  8. Watanabe, K., Ito, S. and Yamamoto, S. Studies on membrane-associated prostaglandin E synthase-2 with reference to production of 12L-hydroxy-5,8,10-heptadecatrienoic acid (HHT). Biochem. Biophys. Res. Commun., 367,782-786, 2008.
  9. Xu, L., Mabuchi, T., Katano, T., Matsumura, S., Okuda-Ashitaka, E., Sakimura, K., Mishina, M. and Ito, S.Nitric oxide (NO) serves as a retrograde messenger to activate neuronal NO synthase in the spinal cord via NMDA receptors. Nitric Oxide, 17, 18-24, 2007.
  10. Takagi, K., Matsumura, S., Okuda-Ashitaka, E., Okuda, K., Watanabe, J., Takahashi, H., Iwakura, Y. and Ito, S. Interleukin-1 is not essential for expression of inducible NOS in hepatocytes induced by lipopolysaccharide in vivo. Nitric Oxide, 16, 433-441, 2007.
  11. Xu L, Okuda-Ashitaka, E., Matsumura, S., Mabuchi T, Okamoto S, Sakimura K, Mishina M, Ito, S. Signal pathways coupled to activation of neuronal nitric oxide synthase in the spinal cord by nociceptin/ orphanin FQ. Neuropharmacology, 52, 1318-1325, 2007.
  12. Ikeura, T., Kataoka, Y., Wakabayashi, T., Mori, T., Takamori, Y., Takamido, S., Okazaki, K. and Yamada, H. Effects of sensory denervation by neonatal capsaicin administration on experimental pancreatitis induced by dibutyltin dichloride. Med. Mol. Morphol., 40, 141-149, 2007.
  13. Katano, T., Mabuchi, T., Okuda-Ashitaka, E., Inagaki, N., Kinumi, T. and Ito, S. Proteomic identification of a novel isoform of collapsin response mediator protein-2 (CRMP-2) in spinal nerves peripheral to dorsal root ganglia. Proteomics, 6, 6085-6094, 2006.
  14. Xu, L., Matsumura, S., Mabuchi, T., Takagi, K., Abe, T. and Ito, S. In situ measurement of neuronal nitric oxide synthase activity in the spinal cord by NADPH-disphorase histochemistry. J. Neurosci. Methods, 150, 174-184, 2006.

６）神経系の再構築・機能修復における細胞接着分子・生理活性物質の役割

  1. Kunori, S., Matsumura, S., Okuda-Ashitaka, E., Katano, T., Audoly, LP., Urade, Y. and Ito, S. A novel role of prostaglandin E2 in neuropathic pain: blockade of microglial migration in the spinal cord. Glia, in press, 2010.
  2. Ebisuno, Y., Katagiri, K., Katakai, T., Ueda, Y., Nemoto, T., Inada, H., Nabekura, J., Okada, T., Kannagi, R., Tanaka, T., Miyasaka, M., Hogg, N. and Kinashi, T. Rap1 controls lymphocyte adhesion cascade and interstitial migration within lymph nodes in RAPL-dependent and -independent manners. Blood, 115, 804-814, 2010.
  3. Kunori, S., Matsumura, S., Mabuchi, T., Tastumi, S., Sugimoto, Y., Minami, T. and Ito, S. Involvement of prostaglandin F2α receptor in ATP-induced mechanical allodynia. Neuroscience, 163, 362-371, 2009.
  4. Katagiri, K., Katakai, T., Ebisuno, Y., Ueda, Y., Okada, T. and Kinashi, T. Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes. EMBO J., 28, 1319-1331, 2009.
  5. Katakai, T., Suto, H., Sugai, M., Gonda, H., Togawa, A., Suematsu, S., Ebisuno, Y., Katagiri, K., Kinashi, T. and Shimizu, A. Organizer-like reticular stromal cell layer common adult secondary lymphoid organs. J. Immunol., 181, 6189-6200, 2008.
  6. Yoshikawa, Y., Satoh, T., Tamura, T., Wei, P., Bilasy, SE., Edamatsu, H., Aiba, A., Katagiri, K., Kinashi, T., Nakao, K. and Kataoka, T. The M-Ras-RA-GEF-2Rap1 pathway mediates tumor-necrosis factor-alpha-dependent regulation of integrin activation in splenocytes. Mol. Biol. Cell, 18, 2949-2959, 2007.
  7. Kinashi, T. Integrin regulation of lymphocyte trafficking: Lessons from structural and signaling studies. Adv. immunol., 93, 185-227, 2007.
  8. Kinashi, T. Adhere upright: A switchblade-like extension of β2 integrins. Immunity, 25, 521-522, 2006.
  9. Katagiri, K., Imamura, M. and Kinashi, T. Spatiotemporal regulation of the Kinase Mst1 by binding protein RAPL is critical for lymphocyte polarity and adhesion. Nat. Immunol., 7, 919-927, 2006.
  10. Okuda-Ashitaka, E., Minami, T., Matsumura, S., Takeshima, H., Reinscheid R.K., Civelli, O. and Ito, S. The opioid peptide nociceptin/orphanin FQ mediates prostaglandin E2-induced allodynia, tactile pain associated with nerve injury. Eur. J. Neurosci., 23, 995-1004, 2006.


II. 神経系の修復・再生機構、変性機構とその予防に関する基礎研究と臨床応用にむけた動物実験

１）アルツハイマー病における変性機構とその予防
  1. Xiong, Z.M., Kitagawa, K., Nishiuchi, Y., Kimura, T., Nakamura, T., and Inagaki, C. Acetyl-Ile-Gly-Leu protects neurons from Aβ1-42-induced toxicity in vitro and in V337M human tau-expressing mice. Life Sci., 84, 132-138, 2009.
  2. Li, M., Inaba, M., Guo, K., Abraham, N.G. and Ikehara, S. Amelioration of cognitive ability in senescence- accelerated mouse prone 8 (SAMP8) by intra-bone marrow-bone marrow transplantation. Neurosci. Lett., 465, 36-40, 2009.
  3. Hattori, N., Kitagawa, K., Nakayama, Y., and Inagaki, C. [Neurotoxicity of amyloid b proteins] (review) Nippon Yakurigaku Zasshi, 131, 326-332, 2008.
  4. Yang, L., Nakayama, Y., Hattori, N., Liu, B. and Inagaki, C. GABAC-receptor stimulation activates cAMP-dependent protein kinase via A-kinase anchoring protein 220. J. Pharmacol. Sci., 106, 578-584, 2008.
  5. Xiong, Z.M., Kitagawa, K., Nishiuchi, Y., Kimura, T., Inagaki, C. Protective effects of Aβ-derived tripeptide, Aβ32-34, on Aβ1-42-induced phosphatidylinositol 4-kinase inhibition and neurotoxicity. Neurosci. Lett., 419, 247-252, 2007.
  6. Zhang, N.Y., Kitagawa, K., Hattori, N., Nakayama, Y., Xiong, Z.M., Wu, B., Liu, B. and Inagaki, C. Soybean-derived phosphatidylinositol inhibits in vivo low concentrations of amyloid β protein-induced degeneration of hippocampal neurons in V337M human tau-expressing mice. Life Sci., 80, 1971-1976, 2007.
  7. Li, T., Nakayama, Y., Kitagawa, K., Hattori, N., Xiong, Z.M., and Inagaki, C. Down-regulation of Cl- pump ClP55 subunit induced enhancement of glutamate neurotoxicity in cultured rat hippocampal neuron. Brain Res., 1130, 235-238, 2007.

２）神経・筋難治性疾患と再生医療

  1. Maruyama, H., Morino, H., Ito, H., Izumi, Y., Kato, H., Watanabe Y., Kinoshita, Y., Kamada, M., Nodera, H., Suzuki, H., Komure, O., Matsuura, S., Kobatake, K., Morimoto, N., Abe, K., Suzuki, N., Aoki, M., Kawata, A., Hirai, T., Kato, T., Ogasawara, K., Hirano, A., Takumi, T., Kusaka, H., Hagiwara, K., Kaji, R. and Kawakami, H. Mutations of optineurin in amyotrophic lateral sclerosis. Nature, 465, 223-226, 2010.
  2. Kawamoto, Y., Ito, H., Kobayashi, Y., Suzuki, Y., Akiguchi, I., Fujimura, H., Sakoda, S., Kusaka, H., Hirano, A. and Takahashi, R. HtrA2/Omi-immunoreactive intraneuronal inclusions in the anterior horn of patients with sporadic and Cu/Zn superoxide dismutase (SOD1) mutant amyotrophic lateral sclerosis. Neuropathol. Appl. Neurobiol., 36, 331-344, 2010.
  3. Munoz, D.G., Neumann, M., Kusaka, H., Yokota, O., Ishihara, K., Terada, S., Kuroda, S. and Mackenzie, I.R. FUS pathology in basophilic inclusion body disease. Acta Neuropathol., 118, 617-627, 2009.
  4. Ohnishi, S., Ito, H., Suzuki, Y., Adachi, Y., Wate, R., Zhang, J., Nakano, S., Kusaka, H., and Ikehara, S. Intra-bone marrow-bone marrow transplantation slows disease progression and prolongs survival in G93A mutant SOD1 transgenic mice, an animal model mouse for amyotrophic lateral sclerosis. Brain Res., 1296, 216-224, 2009.
  5. Kinoshita, Y., Ito, H., Hirano, A., Fujita, K., Wate, R., Nakamura, M., Kaneko, S., Nakano, S. and Kusaka, H. Nuclear Contour Irregularity and Abnormal Transporter Protein Distribution in Anterior Horn Cells in Amyotrophic Lateral Sclerosis. J. Neuropathol. Exp. Neurol., 68, 1184-1192, 2009.
  6. Kamada, M., Maruyama, H., Tanaka, E., Morino, H., Wate, R., Ito, H., Kusaka, H, Kawano, Y., Miki, T., Nodera, H., Izumi, Y., Kaji, R. and Kawakami, H. Screening for TARDBP mutations in Japanese familial amyotrophic lateral sclerosis. J. Neurol. Sci., 284, 69-71, 2009.
  7. Ito, H., Wate, R., Zhang, J., Ohnishi, S., Kaneko, S., Ito, H., Nakano, S. and Kusaka, H. Treatment with edaravone, initiated at symptom onset, slows motor decline and decreases SOD1 deposition in ALS mice. Exp. Neurol., 213, 448-455, 2008.
  8. Fujita, K., Ito, H., Nakano, S., Kinoshita, Y., Wate, R. and Kusaka, H. Immunohistochemical identification of messenger RNA-related proteins in basophilic inclusions of adult-onset atypical motor neuron disease. Acta Neuropathol. (Berl), 116, 439-445, 2008.
  9. Nakamura, M., Ito, H., Wate, R., Nakano, S., Hirano, A., and Kusaka, H. Phosphorylated Smad2/3 immunoreactivity in sporadic and familial amyotrophic lateral sclerosis and its mouse model. Acta Neuropathol., (Berl), 115, 327-334, 2008.
  10. Shinde, A., Nakano, S., Sugawara, M., Toyoshima, I., Ito, H., Tanaka, K. and Kusaka, H. Expression of caveolar components in primary desminopathy. Neuromuscul. Disord., 18, 215-219, 2008.
  11. Nakano, S., Shinde, A., Fujita, K., Ito, H., and Kusaka, H. Histone H1 is released from myonuclei and present in rimmed vacuoles with DNA in inclusion body myositis. Neuromuscul. Disord., 18, 27-33, 2008.
  12. Zhang, J., Ito, H., Wate, R., Ohnishi, S., Nakano, S., and Kusaka, H. Altered distributions of nucleocytoplasmic transport-related proteins in the spinal cord of a mouse model of amyotrophic lateral sclerosis. Acta Neuropathol. (Berl), 112, 673-680, 2006.
  13. Ito, H., Kawakami, H., Wate, R., Matsumoto, S., Imai, T., Hirano, A., and Kusaka, H. Clinicopathologic investigation of a family with expanded SCA8 CTA/CTG repeats. Neurology, 67, 1479-1481, 2006.

３）神経疾患モデル動物を用いる機能性分子の探索

  1. Hamada, S., Houtani, T., Trifonov, S., Kase, M., Maruyama, M., Shimizu, J., Yamashita, T., Tomoda, K. and Sugimoto, T. Histological determination of the areas enriched in cholinergic terminals and m2 and m3 muscarinic receptors in the mouse central auditory system. Anat. Rec., 293, 1393-1399, 2010.
  2. Trifonov, S., Houtani, T., Shimizu, J., Hamada, S., Kase, M., Maruyama, M. and Sugimoto, T. GPR155: Gene organization, multiple mRNA splice variants and expression in mouse central nervous system. Biochem. Biophys. Res. Commun., 398, 9-25, 2010.
  3. Trifonov, S., Houtani, T., Hamada, S., Kase, M., Maruyama, M. and Sugimoto, T. In situ hybridization study of the distribution of choline acetyltransferase mRNA and its splice variants in the mouse brain and spinal cord. Neuroscience, 159, 344-357, 2009.
  4. Tsutsumi, T., Houtani, T., Toida, K., Kase, M., Yamashita, T., Ishimura, K. and Sugimoto, T. Vesicular acetylcholine transporter-immunoreactive axon terminals enriched in the pontine nuclei of the mouse. Neuroscience, 146, 1869-1878, 2007.
  5. Kase, M., Houtani, T., Sakuma, S., Tsutsumi, T. and Sugimoto, T. Laser microdissection combined with immunohistochemistry on serial thin tissue sections: a method allowing efficient mRNA analysis. Histochem. Cell Biol., 127, 215-219, 2007.

４）網膜での細胞死・細胞保護の基礎実験

  1. Ohnaka, N., Okuda-Ashitaka, E., Kaneko, S., Ando, A., Maeda, M., Furuta, F., Suzuki, S., Takahashi, K. and Ito, S. Induction of arginase II mRNA by nitric oxide using in vitro model of gyrate atrophy of choroid and retina. Invest. Ophthalmol. Vis. Sci., in press, 2010.
  2. Kaneko, S., Okuda-Ashitaka, E., Ando, A., Nishimura, K., Igarashi, K., Maeda, M., Furuta, K., Suzuki, M., Matsumura, M. and Ito, S. Polyamines upregulate the mRNA expression of cationic amino acid transporter-1 in human retinal pigment epithelial cells. Am. J. Physiol. Cell Physiol., 293, 729-737, 2007.
  3. Yamazaki, Y., Matsunaga, H., Nishikawa, M., Ando, A., Kaneko, S., Okuda, K., Wada, M., Ito, S. and Matsumura, M. Senescence in cultured trabecular meshwork cells. Br. J. Ophthalmol., 91, 808-811, 2007.
  4. Kaneko, S., Ando, A., Okuda-Ashitaka, E., Maeda, M., Furuta, K., Suzuki, M., Matsumura, M. and Ito, S. Ornithine transport via cationic amino acid transporter-1 is involved in ornithine cytotoxicity in retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci., 48, 464-471, 2007.
  5. Kaneko, S., Ueda-Yamada, M., Ando, A., Matsumura, S., Okuda-Ashitaka, E., Matsumura, M., Uyama, M. and Ito, S. Cytotoxic effect of spermine on retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci., 48, 455-463, 2007.

５）治療に向けた脊髄損傷、脳梗塞モデルの動物実験

  1. Zhen, Y., Zhao, S., Li, Q., Li, Y. and Kawamoto, K. Arsenic trioxide-mediated Notch pathway inhibition depletes the cancer stem-like cell population in gliomas. Cancer Lett., 292, 64-72, 2010.
  2. Li, Q., Oshige, H., Zhen, Y., Yamahara, T., Oishi, T., Seno, T., Kawaguchi, T., Numa, Y. and Kawamoto, K. Interleukin-5 and interleukin-10 are produced in central nervous system tumor cysts. J. Clin. Neurosci., 16, 437-440, 2009.
  3. Li, Q., Hosaka, N., Cui, W., Wang, X., Cui, Y., Cui, Y., Song, C., Li, Q., Ryu, T., Fan, T., Kawamoto, K. and Ikehara, S. Lin-CD34- bone marrow cells from adult mice can differentiate into neural-like cells. Neurosci. Lett., 408, 51-56, 2006.

６）GFP蛍光タンパクを用いた神経修復・再生の可視化

  1. Unezaki, S., Yoshii, S., Mabuchi, T., Saito, A. and Ito, S. Effects of neurotropic factors on nerve regeneration monitored by in vivo imaging in thy1-YFP transgenic mice. J. Neurosci. Methods, 178, 308-315, 2009.
  2. Yoshii, S., Ito, S., Shima, M., Taniguchi, A. and Akagi, A. Functional restoration of rabbit spinal cord using collagen-filament scaffold. J. Tissue Eng. Regen. Med., 3, 19-25, 2009.
  3. Takagi, K., Okuda-Ashitaka, E., Mabuchi, T., Katano, T., Ohnishi, T., Matsumura, S., Ohnaka, M., Kaneko, S., Abe, T., Hirata, T., Fujiwara, S., Minami, T. and Ito, S. Involvement of stem cell factor and its receptor tyrosine kinase c-kit in pain regulation. Neuroscience, 153, 1278-1288, 2008.
  4. Inada, Y., Hosoi, H., Yamashita, A., Morimoto, S., Tatsumi, H., Notazawa, S., Kanemaru, S., and Nakamura, T. Regeneration of peripheral motor nerve gaps with a polyglycolic acid-collagen tube: technical case report. Neurosurgery, 61, E1105-1107, 2007.
  5. Yoshitani, M., Fukuda, S., Itoi, S., Morino, S., Tao, H., Nakada, A., Inada, Y., Endo, K. and, Nakamura, T. Experimental repair of phrenic nerve using a polyglycolic acid and collagen tube. J. Thorac. Cardiovasc. Surg., 133, 726-32, 2007.


III. 組織幹細胞の同定と評価系の確立と治療に向けた動物実験

１）生体における組織幹細胞の同定
  1. Wakabayashi, T., Kosaka, J., Mochii, M., Miki, Y., Mori, T., Takamori, Y. and Yamada, H. C38, equivalent to BM88, is developmentally expressed in maturing retinal neurons and enhances neuronal maturation. J. Neurochem., 112, 1235-1248, 2010.
  2. Matsumura, S., Takagi, K., Okuda-Ashitaka, E., Lu, J., Naritsuka, H., Yamaguchi, M. and Ito, S. Characterization of nestin expression in the spinal cord of GFP transgenic mice after peripheral nerve injury. Neuroscience, 170, 942-953, 2010
  3. Takamori, Y., Mori, T., Wakabayashi, T., Nagasaka, Y., Matsuzaki, T. and Yamada, H. Nestin-positive microglia in adult rat cerebral cortex. Brain Res., 1270, 10-18, 2009.
  4. Mori, T., Wakabayashi, T., Takamori, Y., Kitaya, K. and Yamada, H. Phenotype analysis and quantitatification of proliferating cells in the gray matter of the adult rat. Acta Histochem. Cytochem., 42, 1-8, 2009.
  5. Cui, Y., Kataoka, Y., Inui, T., Mochizuki, T., Onoe, H., Matsumura, K., Urade, Y., Yamada, H. and Watanabe, Y. Up-regulated neuronal COX-2 expression after cortical spreading depression is involved in non-REM sleep induction in rats. J. Neurosci. Res., 86, 929-936, 2008.
  6. Wakabayashi, T., Kosaka, J., Mori, T., Takamori, Y. and Yamada, H. Doublecortin expression continues into adulthood in horizontal cells in the rat retina. Neurosci Lett., 442, 249-252, 2008.
  7. Takamori, Y., Tamura, Y., Kataoka, Y., Cui, Y., Seo, S., Kanazawa, T., Kurokawa, K. and Yamada, H. Differential expression of nuclear lamin, the major component of nuclear lamina, during neurogenesis in two germinal regions of adult rat brain. Eur. J. Neurosci., 25, 1653-1662, 2007.
  8. Tamura, Y., Kataoka, Y., Cui, Y., Takamori, Y., Watanabe, Y. and Yamada, H. Intracellular translocation of glutathione S-transferase pi during oligodendrocyte differentiation in adult rat cerebral cortex in vivo. Neuroscience, 148, 535-540, 2007.
  9. Tamura, Y., Kataoka, Y., Cui, Y., Takamori, Y., Watanabe, Y. and Yamada, H. Multi-directional differentiation of doublecortin- and NG2-immunopositive progenitor cells in the adult rat neocortex in vivo. Eur. J. Neurosci., 25, 3489-3498, 2007.

２）血球系幹細胞の同定とシグナル伝達

  1. Moro, K., Yamada, T., Tanabe, M., Takeuchi, T., Ikawa, T., Kawamoto, H., Furusawa, JI., Ohtani, M., Fujii, H. and Koyasu, S. Innate production of TH2 cytokines by adipose tissue-associated c-Kit+Sca-1+ lymphoid cells. Nature, 463, 540-544, 2010.
  2. Nakatsuka, R., Nozaki, T., Uemura, Y., Matsuoka, Y., Sasaki, Y., Shinohara, M., Ohura, K. and Sonoda, Y. 5-Aza-2’-deoxycytidine treatment induces skeletal myogenic differentiation of mouse dental pulp stem cells. Arch. Oral Biol., 55, 350-357, 2010.
  3. Kimura, T., Matsuoka, Y., Murakami, M., Kimura, T., Takahashi, M., Nakamoto, T., Yasuda, K., Matsui, K., Kobayashi, K., Imai, S., Asano, H., Nakatsuka, R., Uemura, Y., Sasaki, Y. and Sonoda, Y. In vivo dynamics of human cord blood-derived CD34- SCID-repopulating cells using intra-bone marrow injection. Leukemia, 24, 162-168, 2010.
  4. 薗田精昭. ヒト未分化CD34抗原陰性造血幹細胞の純化とその幹細胞特性, 血液・腫瘍科, in press, 2010.
  5. Matsuda, S., Mikami, Y., Ohtani, M., Fujiwara, M., Hirata, Y., Minowa, A., Terauchi, Y., Kadowaki, T. and Koyasu, S. Critical role of class IA PI3K for c-Rel expression in B lymphocytes. Blood, 113, 1037-1044, 2009.
  6. Sasaki, Y., Matsuoka, Y., Hase, M., Toyohara, T., Murakami, M., Takahashi, M., Nakatsuka, R., Uemura, Y. and Sonoda, Y. Marginal expression of CXCR4 on c-kit+Sca-1+Lineage- hematopoietic stem/progenitor cells. Int. J. Hematol., 90, 553-560,2009.
  7. Sonoda, Y. Immunophenotype and functional characteristics of human primitive CD34-negative hematopoietic stem cells: The significance of the intra-bone marrow injection. J. Autoimmun., 30, 136-144, 2008.
  8. Ohtani, M., Nagai, S., Kondo, S., Mizuno, S., Nakamura, K., Tanabe, M., Takeuchi, T., Matsuda, S. and Koyasu, S. mTOR and GSK3 differentially regulate LPS-induced IL-12 production in dendritic cells. Blood, 112, 635-643, 2008.
  9. Fujii, Y., Matsuda, S., Takayama, G. and Koyasu, S. ERK5 is involved in TCR-induced apoptosis through the modification of Nur77. Genes Cells, 13, 411-419, 2008.
  10. 薗田精昭. 造血幹細胞の骨髄内移植法―その臨床的意義―, 最新医学, 63, 2311-2317. 2008.
  11. Kimura, T., Asada, R., Wang, J., Kimura, T., Morioka, M., Matsui, K., Kobayashi, K., Henmi, K., Imai, S., Kita, M., Tsuiji, T., Sasaki, Y., Ikehara, S. and Sonoda, Y. Identification of long-term repopulating potential of human cord blood-derived CD34-flt3- severe combined immunodeficiency- repopulating cells by intra-bone marrow injection. Stem Cells, 25, 1348-1355, 2007.
  12. Harada, S., Kimura, T., Fujiki, H., Nakagawa, H., Ueda, Y., Itoh, T., Yamagishi, H. and Sonoda, Y. Flt3 ligand promotes myeloid dendritic cell differentation of human hematopoietic progenitor cells: Possible application for cancer immunotherapy. Int. J. Oncol., 30, 1461-1468, 2007.
  13. Shiroki, F., Matsuda, S., Doi, T., Fujiwara, M., Mochizuki, Y., Kadowaki, T., Suzuki, H. and Koyasu, S. The p85α regulatory subunit of class IA phosphoinositide 3-kinase regulates β-selection in thymocyte development. J. Immunol., 178, 1349-1356, 2007.

３）修復・再生過程における幹細胞を追跡できるウイルスベクターの開発

  1. Yanai, S., Adachi, Y., Fujisawa, J., Jiang, S., Okigaki, M., Shi, M., Shima, C., Imai, Y., Kwon, A.H. and Ikehara, S. Anti-tumor effects of fusion cells of type 1 dendritic cells and Meth A tumor cells using hemagglutinating virus of Japan-envelope. Int. J. Oncol., 35, 249-255, 2009.
  2. Jiang, S., Inada, T., Tanaka, M., Furuta, R.A., Shingu, K. and Fujisawa, J. Involvement of TORC2, a CREB co-activator, in the in vivo-specific transcriptional control of HTLV-1. Retrovirology, 6, 73 2009.
  3. Sugiura, K., Taketani, S., Yoshimura, T., Nishino, T., Nishino, N., Fujisawa, J., Hisha, H., Inaba, T. and Ikehara, S. Effect of hepatocyte growth factor on long term hematopoiesis of human progenitor cells in transgenic-severe combined immunodeficiency mice. Cytokine, 37, 218-226 2007.
  4. Yasui, K., Miyazaki, T., Matsuyama, N., Kojima, Y., Furuta, R. A., Fujisawa, J., Tani, Y., Shibata, H., Sato, S., Kato, T., Ikeda, H. and Hirayama, F. Establishment of cell lines stably expressing HNA-1a, -1b, and -2a antigen with low background reactivity in flow cytometric analysis. Transfusion, 47, 478-485 2007.
  5. Furuta, R.A., Nishikawa, M. and Fujisawa, J-i. Real-time analysis of human immunodeficiency virus type 1 Env-mediated membrane fusion by fluorescence resonance energy transfer. Microbes Infect., 8, 520-532, 2006.

４）遺伝子導入による内耳有毛細胞の再生と機能回復

  1. Kawamoto, K., Izumikawa, M., Beyer, L.A., Atkin, G.M. and Raphael, Y. Spontaneous hair cell regeneration in the mouse utricle following gentamicin ototoxicity. Hear. Res., 247, 17-26, 2008.
  2. Konishi, M., Kawamoto, K., Izumikawa, M., Kuriyama, H. and Yamashita, T. Gene transfer into guinea pig cochlea using adeno-associated virus vectors. J. Gene Med., 10, 610-618, 2008.
  3. Izumikawa, M, Batts, S, Miyazawa, T., Swiderski, D. and Raphael, Y. Response of the flat cochlear epithelium to forced expression of Atoh1. Hear. Res., 240, 52-56, 2008.
  4. Raphael, Y., Kim, Y.H., Osumi, Y. and Izumikawa, M. Non-sensory cells in the deafened organ of Corti: approaches for repair. Int. J. Dev. Biol., 51, 649-654, 2007.
  5. Minoda, R., Izumikawa, M., Kawamoto, K., Zhang, H. and Raphael, Y. Manipulating cell cycle regulation in the mature cochlea. Hear. Res., 232, 44-51, 2007.
  6. Mustapha, M., Beyer, L., Izumikawa, M., Swiderski, D., Dolan, D., Raphael, Y. and Camper, S. Whirler mutant hair cells have less severe pathology than shaker 2 or double mutants. J. Assoc. Res. Otolaryngol., 8, 329-337, 2007.
  7. Abrashkin, K.A., Izumikawa, M., Miyazawa, T., Wang, C.H., Crumling, M.A., Swiderski, D.L., Beyer, L.A. and Raphael, Y. The fate of outer hair cells after acoustic or ototoxic insults. Hear. Res., 218, 20-29, 2006.
  8. Kanzaki, S., Beyer, L.A., Swiderski, D.L., Izumikawa, M., Stover, T., Kawamoto, K. and Raphael, Y. p27(Kip1) deficiency causes organ of Corti pathology and hearing loss. Hear. Res., 214, 28-36, 2006.
  9. Shen, J., Harada, N., Nakazawa, H., Kaneko, T., Izumikawa, M. and Yamashita, T. Role of nitric oxide on ATP-induced Ca2+ signaling in outer hair cells of the guinea pig cochlea. Brain Res., 1081, 101-112, 2006.
  10. 河本光平, 小西将矢, 栗山博道. アデノ随伴ウィルスベクターを用いた内耳への遺伝子導入, 逓信医学, 59, 185-189, 2007.
  11. 河本光平, 小西将矢, 栗山博道. 内耳への遺伝子導入, 逓信医学, 58, 263-266, 2006.

５）椎間板再生における骨髄細胞の効果と移植の基礎実験

  1. Okamoto, N., Kushida, T., Oe, K., Umeda, M., Ikehara, S. and Iida, H. New strategy for treating Achilles tendon rupture in rats using bone marrow cell transplantation therapy. J. Bone Joint Surg., in press, 2010.
  2. Umeda, M., Kushida, T., Sasai, K., Asada, T., Oe, K., Sakai, D., Mochida, J., Ikehara, S. and Iida, H. Activation of rat nucleus pulposus cells by coculture with whole bone marrow cells collected by the perfusion method. J. Orthop. Res., 27, 222-228, 2009.

６）骨髄間質細胞を用いた急性期脊髄損傷治療

  1. Ide, C., Nakai, Y., Nakano, N., Seo, T.B., Yamada, Y., Endo, K., Noda, T., Saito, F., Suzuki, Y., Fukushima, M. and Nakatani, T. Bone marrow stromal cell transplantation for treatment of sub-acute spinal cord injury in the rat. Brain Res., 1332, 32-47, 2010.
  2. Kato, H., Kimura, A., Sasaki, R., Kaneko, N., Takeda, M., Hagiwara, A., Ogura, S., Mizoguchi, T., Matsuoka, T., Ono, H., Matsuura, K., Matsushima, K., Kushimoto, S., Fuse, A., Nakatani, T., Iwase, M., Fudoji, J. and Kasai, T. Cervical spinal cord injury without bony injury: A multicenter retrospective study of emergency and critical care centers in Japan. J. Trauma, 65, 373-379, 2008.
  3. Saito, F., Nakatani, T., Iwase, M., Maeda, Y., Hirakawa, A., Murao, Y., Suzuki, Y., Onodera, R., Fukushima, M. and Ide, C. Spinal cord injury treatment with intrathecal autologous bone marrow stromal cell transplantation: The first clinical trial case report. J. Trauma, 64, 53-59, 2008.
  4. 中谷壽男. 脊髄損傷に対する自己骨髄間質細胞の髄液内投与による治療, Clin. Eng., 20, 19-26, 2009.
  5. 中谷壽男. 神経再生臨床応用：骨髄間質細胞による脊髄損傷の治療. 脳21, 12, 322-327, 2009.
  6. 齊藤福樹, 中谷壽男, 岩瀬正顕. 急性期脊髄損傷に対する自家骨髄間質細胞移植. Jap. J. Rehabil. Med., 45, 349-364, 2008.
  7. 中谷壽男. 脊髄損傷再生臨床試験について: 関西医科大学高度救命救急センターから, 脊椎脊髄ジャーナル, 20, 1263-1267, 2007.
  8. 中谷壽男. 脊髄損傷の再生治療, 救急医学, 31, 1785-1789, 2007.
  9. 中谷壽男, 鈴木義久, 井出千束, 福島雅典. 脊髄損傷の再生医療, 臨床スポーツ医学, 23, 1081-1085, 2006.

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◆ 図　書 ◆
  1. 薗田精昭 (日本医事新報社) 造血幹細胞の本体はどこまで解明されたのか.「造血器腫瘍アトラス」、17-33, 2009.
  2. 薗田精昭 (日本医事新報社) 骨髄内造血幹細胞移植.「造血器腫瘍アトラス」、538-543, 2009.
  3. 片野泰代, 伊藤誠二 (三輪書店)　慢性痛における生化学的変化.「疼痛の理学療法―慢性痛の理解とエビデンス 第2版」、31-40, 2008.
  4. 中井吉英, 阿部哲也, 水野泰行 (真興交易) 心身症としての慢性疼痛の病態. 「慢性疼痛の理解と医療連携」、 35-45, 2008.
  5. 伊藤誠二, 大西隆之 (真興交易)　慢性疼痛の発生・維持の機序. 「慢性疼痛の理解と医療提携」、57-69, 2008.
  6. Sonoda, Y (Elsevier) The use of bone marrow transplantation to treat autoimmune disease. Immunophenotype and functional characteristics of human primitive CD34-negative hematopoietic stem cells: The significance of the intra-bone marrow injection. 136-144, 2008.
  7. 薗田精昭. (科学評論社) 幹細胞と再生医学. 「造血幹細胞移植のすべて: 血液・腫瘍科特別増刊号」、55, 52-62, 2007.
  8. 町田英世, 中井吉英. (先端医学社) SSRIによる身体症状・疾患へのアプロ−チ: 慢性疼痛.「SSRIのすべて」、162-167, 2007.
  9. Ando, A., Kaneko, S., Kiriyama, N., Unezaki, S., Okuda-Ashitaka, E., Okumura, T., Nishimura, N., Matsumura, M. and Ito, S. (Nova Sience Publishers) Prevention of apoptosis by down-regulation of apoptosis related gene expression obtained by nipradilol via nitric oxide donative action. “Cell Apoptosis-Regulation and Environmental Factors” 55-69, 2006.

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◆ 学 会 発 表 ◆
  1. Kinashi, T. Regulation of lymphocyte trafficking by RAPL and MST1 signaling. The 2009 FASEB (Federation of American Societies for Experimental Biology)Summer Research Conference,Signal Transduction in The Immune System. Snowmass, U.S.A., 2009/06.
  2. Saito, F., Nakatani, T., Iwase, M., Hirakawa, A., Murao, Y., Matsumoto, K., Sakamoto, T., Suzuki, Y. and Ide, C. Two-year follow-up of the effects of intrathecal bone marrow stromal cell administration treatment in a patient with spinal cord injury. American Association for the Surgery of Trauma 67th Meeting, Maui, U.S.A., 2008/09.
  3. Ito, S. Characterizaiton of signaling pathways for the translocation of neuronal nitric oxide synthase to the plasma membrane by pituitary adenylate cyclase-activating polypeptide (PACAP). The 3rd Asian Pain Symposium, Fukuoka, 2008/07.
  4. Nakamura, K. Neuronal Activity of the Primate Dorsal Raphe Nucleus in the Biased Reward Saccade Tasks. Society for Neuroscience Meeting, Atlanta, U.S.A., 2007/11.
  5. Nakamura, K. Differential reward coding by dorsal raphe neurons and dopamine neurons. Champalimaud Neuroscience Workshops: Instituto Gulbenkian de Ciencia, Oeiras, Portugal, 2007/10.
  6. Nakatani, T., Saito, F., Iwase, M., Suzuki, Y., Hirakawa, A., Murao, Y. and Ide, C. Spinal Cord Regeneration with bone marrow stromal cell administration in the cerebro-spinal fluid: Report of the first case at 6 months． American Association for the Surgery of Trauma 66th Meeting, Las Vegas, U.S.A., 2007/09
  7. Kinashi, T., Katagiri, K. and Ebisuno, Y. The Rap1-RAPL-Mst1 signaling. Gordon Research Conference: Mechanisms of Cell Signaling, Oxford, U.K., 2007/9.
  8. Houtani, T., Toida, K., Tsutsumi, T., Kase, M., Yamashita, T., Ishimura, K. and Sugimoto, T. CharActarization of cholinergic terminal-like structures in the mouse pontine nuclei. 7th IBRO World Congress of Neuroscience, Melbourne, Australia, 2007/07.
  9. Umeda, M., Kushida., Sasai, K., Asada, T. and Iida, H. Activation of Nucleus Pulposus Cells by Co-Culturing with Whole Bone Marrow Cells Collected by Perfusion Method in Rats. World Forum for Spine Research-The Intervertebral Disc, Kyoto, 2007/02.
  10. Ito, H., Kawakami, H., Wate, R., Shinde, A., Nakano, S., Matsumoto, S., Imai, T., Hirano, A., and Kusaka, H. Clinical and neuropathologic investigation of a family with the CTA/CTG expansions on SCA8 allele. 16th International Congress of Neuropathology, San Francisco, U.S.A., 2006/09.

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